Iz models dating
These maps anchor the fingerprint-based contig assemblies to chromosome location.
High-Molecular-Weight DNA was prepared by Jack Gardiner in the lab at Missouri and shipped to Clemson (Wing's lab at the time) and to de Jong's lab (just at the time his lab was moving to California) for BAC preparation.
When requesting seed from the North Central Regional Plant Introduction Station, ask for any lot descended from the Coe PI 550473 lines.
The stock was received directly by the North Central Regional Plant Intoduction Station from Arnel Hallauer and has been maintained by the quality-maintenance procedures at the PI Station.
NSF grant reports have documented the details, and specifics for the materials, preparation, characterization, and final assembly of the contig framework can be found in Coe E, Schaeffer ML (2005) Genetic, physical, maps, and database resources for maize. Ed Coe has made a copy of that paper available here What is a Reference Genome? What are the main changes between Ref Gen_v2 and Ref Gen_v3?
How can I map positions between the v2 and v3 assemblies?
More complete details in the B73Ref Gen_v4 assembly can be found at Gramene or by reading the paper. Next assembly version: The release date of the next assembly update is not known at this time (October 2018) Release dates will be posted here and elsewhere at Maize GDB as they become available.Ed Coe reports that, "The results of QC lab checks for constancy in PI 550473 have been excellent." The same source was used for the IBM mapping population.Maps produced at Missouri used 302 lines of this population, providing unmatched precision (resolution is at the intra-BAC level).Where can I find legacy resources from Maize Sequence. How can I identify the Filtered Gene Set (FGS) in Ref Gen_v3?Where can I download a GFF dump of the FGS for maize genes in v3 (5b )? A Reference Genome is a haploid representation of a genome as DNA sequence with a defined coordinate system, and accession and version identification.
Due to the difficulty of determining when two gene models are the same (or when one represents an alternative splicing of the same genomic material), there are no plans to merge the sets.